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Transmission of malaria is common in countries with tropical and subtropical climates. In several cases, this disease presents in the sufferer without symptoms, which is asymptomatic, especially in individuals that have never received treatment shortly. This study aimed to determine the prevalence of this condition by RDT, Giemsa staining, and Nested PCR in Anak Dalam Tribe (ADT), Jambi, Indonesia. This descriptive research with a survey design was conducted on 99 persons in ADT. It was discovered that majority of the respondents were the adult age category (52.5%), with female sex (50.5%). The prevalence of this infectious disease as detected by RDT, microscopy, and Nested PCR examination were 3%, 5% and 35.3% respectively. Non-Plasmodium falciparum was detected by examining samples with RDT method, on microscope, P. vivax and P. falciparum were found, while on Nested PCR, two bands at 120bp and 205bp showed P. vivax and P. falciparum respectively. To reduce morbidity and mortality resulting from malaria, authorized health service centers should regularly conduct screening with RDT and microscopic examinations of vulnerable age groups and pregnant women.
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